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  • miRNA的检测
    miRNA的检测
    Real-time PCR方法克服了由于miRNA分子太短（~22 nt）带来的定量最大难题而引入靶向特异性的反转录引物，该RT引物可以与成熟miRNA结合，形成反转录引物/成熟miRNA复合物，并在miRNA的5’末端延伸。这样就得到一个较长的反转录扩增因子，为进一步做实时定量PCR提供了符合要求的摸板。
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  • 荧光定量PCR
    荧光定量PCR
    实时荧光定量PCR（Real Time PCR）技术，是指在PCR指数扩增期间通过连续监测荧光信号强弱的变化来即时测定特异性产物的量，最后通过标准曲线对未知模板进行定量分析的方法。
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  • 化学合成miRNA
    化学合成miRNA
    近年来人工合成的miRNA（artificial miRNA，amiRNA）已经成功应用于沉默预期靶基因的表达及其功能研究，人工合成的miRNAs既能够特异性地沉默单一基因，也可以同时沉默多个相关但不相同的基因。miRNA产品主要包括miRNA mimics和miRNA inhibitor。
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  • 基因克隆
    基因克隆
    每次克隆需5~7个工作日，并依据难度收取相应的克隆费用；基因定点突变和克隆服务，如果客户提供的模板是PCR产物，需另外收取TA克隆测序的费用
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  • 基因组步移
    基因组步移
    基因组步移
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  • 基因调取
    基因调取
    总RNA提取、反转录、PCR扩增、测序或克隆测序
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  • 引物合成
    引物合成
    安诺伦生物DNA合成服务已有多年专业DNA化学合成的历史，在DNA化学合成中积累了丰富的经验，可满足如PCR扩增、测序以及基因合成等不同实验需求。安诺伦生物采用国际先进的DNA合成仪，配以熟练的专业技术人员，无论哪种DNA合成项目，安诺伦均可进行100%质谱检测，确保引物没有缺失，序列更加准确。
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  • 密码子优化
    密码子优化
    编码相同氨基酸的不同密码子，在不同物种、不同生物体中使用的频率的不平均分布，也即绝大多数生物倾向于只使用64种密码子中的一部分，这种现象称为密码子的偏好性。每种用做蛋白表达或生产的生物（包括大肠杆菌，酵母，哺乳动物细胞，Pichia，植物细胞和昆虫细胞）都表现出某种程度的密码子使用的差异或偏好。
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  • 基因组DNA的提取
    基因组DNA的提取
    基因组DNA的提取
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  • RNA提取及反转录
    RNA提取及反转录
    RNA提取及反转录
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  3. 缓存信息 : 0 reads,0 writes
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