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产品描述
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Certainly! Below is a structured product information guide for the EZ DNA Methylation Kit, covering the aspects you mentioned.
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EZ DNA Methylation Kit
Storage:
- Store the kit components at room temperature upon receipt.
- Ensure that the reagents are kept in a cool, dry place away from direct sunlight.
- For long-term storage, it is recommended to keep sensitive components, such as the conversion reagent, at 4°C to maintain their efficacy.
Product Description:
The EZ DNA Methylation Kit is designed for the efficient conversion of unmethylated cytosines to uracil in DNA samples, facilitating downstream methylation analysis. This kit streamlines the bisulfite conversion process, enabling researchers to investigate DNA methylation patterns with high precision. It is suitable for a variety of sample types including genomic DNA from tissues, cells, and FFPE samples.
Key Features:
- High Efficiency: Achieves over 99% conversion of cytosine residues without degradation of DNA.
- Simplicity: User-friendly protocol with minimal handling steps.
- Speed: Conversion process can be completed in less than three hours.
- Compatibility: Suitable for a wide range of DNA inputs, from nanogram to microgram quantities.
- Flexibility: Compatible with multiple downstream applications including PCR, sequencing, and array-based techniques.
What's Included:
- Conversion Reagent: Specially formulated for efficient conversion of cytosines to uracil.
- Binding Buffer: Facilitates binding of converted DNA to the provided spin columns.
- Wash Buffer: Ensures removal of impurities and unreacted reagents.
- Spin Columns: Designed for optimal recovery of bisulfite-converted DNA.
- Collection Tubes: Used for elution and storage of processed DNA.
- Elution Buffer: Preserves the integrity of modified DNA for downstream applications.
- Detailed Protocol: Step-by-step instructions for successful conversion and purification.
Applications:
- DNA Methylation Analysis: Ideal for studying methylation patterns in genes and regulatory regions.
- Epigenetic Research: Supports investigations into epigenetic modifications and their role in gene expression and disease.
- Cancer Research: Useful in identifying methylation biomarkers for cancer diagnostics and prognostics.
- Developmental Biology: Facilitates studies on epigenetic changes during development.
- Pharmacogenomics: Assists in understanding methylation-based drug responses and resistance.
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