Certainly! Here's a comprehensive overview of DMEM (Dulbecco's Modified Eagle Medium) with high glucose, supplemented with detailed information across various aspects:


Storage
- Conditions: Store DMEM (high glucose) at 2-8°C. Protect from light to prevent degradation.
- Shelf Life: Typically, unopened DMEM can be stored for up to 12 months. Once opened, use within 4-6 weeks if stored properly.


Product Description
DMEM (high glucose) is a widely utilized cell culture medium designed to support the growth and maintenance of various mammalian cells. The high glucose formulation (4.5 g/L) provides additional energy, making it ideal for fast-growing cells or those requiring extra metabolic support.


Key Features
- High Glucose Concentration: Provides enhanced energy for vigorous cell growth and proliferation.
- Balanced Salt Solution: Ensures appropriate osmotic balance and provides essential ions for cellular function.
- Buffer System: Contains sodium bicarbonate for maintaining physiological pH levels.
- Enriched with Vitamins and Amino Acids: Supports overall cell health and function.
- Versatile Formulation: Suitable for a variety of cell types, including fibroblasts, endothelial cells, and primary cells.


What's Included
- DMEM (High Glucose) Medium: Provided in sterile, sealed bottles. Available in various volumes, such as 500 mL and 1 L.
- Certificate of Analysis: Details the quality and specifications of the batch.
- User Guide: Instructions for optimal use and handling.


Applications
- Cell Culture: Ideal for the cultivation of mammalian cells, including primary cells and established cell lines.
- Transfection Experiments: Supports cell health during gene delivery processes.
- Hybridoma Production: Used in the generation and maintenance of hybridoma cells for monoclonal antibody production.
- Metabolic Studies: Suitable for experiments requiring high energy input due to elevated glucose levels.
- Tissue Engineering: Provides a nutrient-rich environment for the development and maintenance of tissue constructs.

By ensuring proper storage and understanding the product's features and applications, researchers can maximize the efficacy and reliability of DMEM (high glucose) in their experimental workflows.